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• If Internal Control (IC) was added during extraction
Annealing/Extension 60 °C 1:00 (fluorescence read during this step) Fluorescence settings Reporter dye: FAM
① If the blood volume of umbilical cord blood (including anticoagulant) is less than 80mL or the expected NK harvest is more
40 cycles of 95°C 0:15 and 60°C 1:00 with signal read at 60°C
Human SCF Package Size:1mg • If Internal Control (IC)Bcakground Stem cell factor (SCF)is a potent hematopoietic growth factor required in regulating both embryonic and adult hematopoiesis. SCF protein promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). And SCF assists in the recovery of cardiac
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