Human GM-CSF ELISA Kit Package Size:96T 002 pg/µL depending on preparation

002 pg/µL depending on preparation Reaction volume 20 µL total reaction volume (15 µL qRT-PCR mix + 5 µL sample or control) Sample input volume 5 µL purified sample (or 5 µL standard/NTC/NCS) per 20 µL reaction Thermal cycling conditions Reverse transcription: 50 °C for 15:00 (1 cycle)

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Each tube contains a nominal 10 CFU

5 pg/mL – 4000 pg/mL Recovery 82% – 96% Average Recovery 90% Intra Precision 4

Human GM-CSF ELISA Kit Package Size:96T 002 pg/µL depending on preparationSpecifications Name Human GM CSF ELISA Kit Hgnc ID 2434 Specie human Assay Type sandwich Assay Time 3. 5 h Sample Type Serum, plasma, cell culture supernatant, and other biological samples Sample Amount 20 L Sensitivity 4. 76 pg mL Range Standard 1. 56 pg mL 100 pg mL Recovery 97% 110% Average Recovery 104% Intra Precision 2. 5% 2. 9% Inter Precision 3. 7% 4. 9% Plate Type Detachable 96 well plate Platform ELISA Components 96 well polystyrene enzyme