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When using ValidPrime® only m + n + 1 controls are needed
The primers have been designed to be exon-spanning where possible
TATAA Probe GrandMaster® mix
The bias is NOT introduced when using the “all samples” or “all assays” plate layout or when performing ΔΔCq based analysis
CRISPR-Test Assay Kits When using ValidPrime® only mAccurately Assess Cas9 Activity in Cells Measure functional activity of Cas9 nuclease, dCas9 repressor, or dCas9 activator in any cell line Obtain quantitative FACS based read outs Get complete, easy to use kits with ready to transduce lentiviral particles The CRISPR Test assay kits contain a mix of lentiviral particles with both green fluorescent and red fluorescent protein (GFP and RFP). The GFP fluorescent marker responds to the specific Cas9
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